Stepwise requirements for polymerases δ and θ in theta-mediated end joining

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  • Ramsden, D. A., Carvajal-Garcia, J. & & Gupta, G. P. Mechanism, cellular functions and cancer functions of polymerase-theta-mediated DNA end signing up with. Nat. Rev. Mol. Cell Biol. 23, 125– 140 (2022 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Minocherhomji, S. et al. Duplication tension triggers DNA repair work synthesis in mitosis. Nature 528, 286– 290 (2015 ).

    Article
    CAS
    PubMed
    ADS

    Google Scholar

  • Costantino, L. et al. Break-induced duplication repair work of harmed forks causes genomic duplications in human cells. Science 343, 88– 91 (2014 ).

    Article
    CAS
    PubMed
    ADS

    Google Scholar

  • Kent, T., Chandramouly, G., McDevitt, S. M., Ozdemir, A. Y. & & Pomerantz, R. T. Mechanism of microhomology-mediated end-joining promoted by human DNA polymerase theta. Nat. Struct. Mol. Biol. 22, 230– 237 (2015 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Wyatt, D. W. et al. Necessary functions for polymerase theta-mediated end participating the repair work of chromosome breaks. Mol. Cell 63, 662– 673 (2016 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Yousefzadeh, M. J. et al. System of suppression of chromosomal instability by DNA polymerase POLQ. PLoS Genet. 10, e1004654 (2014 ).

    Article
    PubMed
    PubMed Central

    Google Scholar

  • Carvajal-Garcia, J. et al. Mechanistic basis for microhomology recognition and genome scarring by polymerase theta. Proc. Natl Acad. Sci. U.S.A. 117, 8476– 8485 (2020 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Eckstein, F. Nucleoside phosphorothioates. Annu. Rev. Biochem. 54, 367– 402 (1985 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Arana, M. E., Seki, M., Wood, R. D., Rogozin, I. B. & & Kunkel, T. A. Low-fidelity DNA synthesis by human DNA polymerase theta. Nucleic Acids Res. 36, 3847– 3856 (2008 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Schmitt, M. W. et al. Active website anomalies in mammalian DNA polymerase delta change precision and duplication fork development. J. Biol. Chem. 285, 32264– 32272 (2010 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Weedon, M. N. et al. An in-frame removal at the polymerase active website of POLD1 triggers a multisystem condition with lipodystrophy. Nat. Genet. 45, 947– 950 (2013 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Hu, Z., Perumal, S. K., Yue, H. & & Benkovic, S. J. The human lagging hair DNA polymerase delta holoenzyme is distributive. J. Biol. Chem. 287, 38442– 38448 (2012 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Lancey, C. et al. Structure of the processive human Pol delta holoenzyme. Nat. Commun. 11, 1109 (2020 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Fleury, H. et al. The APE2 nuclease is necessary for DNA double-strand break repair work by microhomology-mediated end signing up with. Mol. Cell 83, 1429– 1445. e8 (2023 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Bennardo, N., Cheng, A., Huang, N. & & Stark, J. M. Alternative-NHEJ is a mechanistically unique path of mammalian chromosome break repair work. PLoS Genet. 4, e1000110 (2008 ).

    Article
    PubMed
    PubMed Central

    Google Scholar

  • Hedglin, M., Pandey, B. & & Benkovic, S. J. Stability of the human polymerase delta holoenzyme and its ramifications in lagging hair DNA synthesis. Proc. Natl Acad. Sci. U.S.A. 113, E1777– E1786 (2016 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Hussmann, J. A. et al. Mapping the hereditary landscape of DNA double-strand break repair work. Cell 184, 5653– 5669 e5625 (2021 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Schimmel, J., Kool, H., van Schendel, R. & & Tijsterman, M. Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. EMBO J. 36, 3634– 3649 (2017 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Feng, W. et al. Marker-free metrology of repair work path usage at Cas9-induced double-strand breaks. Nucleic Acids Res. 49, 5095– 5105 (2021 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Lee, K. & & Lee, S. E. Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA development at DNA break promotes microhomology-mediated end signing up with. Genetics 176, 2003– 2014 (2007 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Meyer, D., Fu, B. X. & & Heyer, W. D. DNA polymerases delta and lambda comply in fixing double-strand breaks by microhomology-mediated end-joining in Saccharomyces cerevisiae Proc. Natl Acad. Sci. U.S.A. 112, E6907– E6916 (2015 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Villarreal, D. D. et al. Microhomology directs varied DNA break repair work paths and chromosomal translocations. PLoS Genet. 8, e1003026 (2012 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Takata, K. I. et al. Analysis of DNA polymerase nu function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance. PLoS Genet. 13, e1006818 (2017 ).

    Article
    PubMed
    PubMed Central

    Google Scholar

  • Mann, A. et al. POLtheta avoids MRE11-NBS1-CtIP-dependent fork damage in the lack of BRCA2/RAD51 by filling lagging-strand spaces. Mol. Cell 82, 4218– 4231 e4218 (2022 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Mengwasser, K. E. et al. Hereditary screens expose FEN1 and APEX2 as BRCA2 artificial deadly targets. Mol. Cell 73, 885– 899 e886 (2019 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Boboila, C. et al. Robust chromosomal DNA repair work by means of alternative end-joining in the lack of X-ray repair work cross-complementing protein 1 (XRCC1). Proc. Natl Acad. Sci. U.S.A. 109, 2473– 2478 (2012 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Masani, S., Han, L., Meek, K. & & Yu, K. Redundant function of DNA ligase 1 and 3 in alternative end-joining throughout immunoglobulin class switch recombination. Proc. Natl Acad Sci. U.S.A. 113, 1261– 1266 (2016 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Mateos-Gomez, P. A. et al. Mammalian polymerase theta promotes alternative NHEJ and reduces recombination. Nature 518, 254– 257 (2015 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Burgers, P. M. Polymerase characteristics at the eukaryotic DNA duplication fork. J. Biol. Chem. 284, 4041– 4045 (2009 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Levin, D. S., McKenna, A. E., Motycka, T. A., Matsumoto, Y. & & Tomkinson, A. E. Interaction in between PCNA and DNA ligase I is important for signing up with of Okazaki pieces and long-patch base-excision repair work. Curr. Biol. 10, 919– 922 (2000 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Fan, J., Otterlei, M., Wong, H. K., Tomkinson, A. E. & & Wilson, D. M. 3rd XRCC1 co-localizes and physically connects with PCNA. Nucleic Acids Res. 32, 2193– 2201 (2004 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Deshpande, M. et al. Error-prone repair work of stalled duplication forks drives mutagenesis and loss of heterozygosity in haploinsufficient BRCA1 cells. Mol. Cell 82, 3781– 3793 e3787 (2022 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Llorens-Agost, M. et al. POLtheta-mediated end signing up with is limited by RAD52 and BRCA2 up until the beginning of mitosis. Nat. Cell Biol. 23, 1095– 1104 (2021 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Roerink, S. F., van Schendel, R. & & Tijsterman, M. Polymerase theta-mediated end signing up with of replication-associated DNA breaks in C. elegans Genome Res. 24, 954– 962 (2014 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • van Schendel, R., Romeijn, R., Buijs, H. & & Tijsterman, M. Preservation of lagging hair stability at websites of stalled duplication by Pol alpha-primase and 9-1-1 complex. Sci. Adv. 7, eabf2278 (2021 ).

    Article
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Wang, Z. et al. DNA polymerase theta (POLQ) is necessary for repair work of DNA double-strand breaks triggered by fork collapse. J. Biol. Chem. 294, 3909– 3919 (2019 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Belan, O. et al. POLQ seals post-replicative ssDNA spaces to preserve genome stability in BRCA-deficient cancer cells. Mol. Cell 82, 4664– 4680 e4669 (2022 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Heijink, A. M. et al. Sis chromatid exchanges caused by irritated duplication can form individually of BRCA1, rad51 and brca2. Nat. Commun. 13, 6722 (2022 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Schrempf, A. et al. POLtheta procedures ssDNA spaces and promotes duplication fork development in BRCA1-deficient cells. Cell Rep. 41, 111716 (2022 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Donnianni, R. A. et al. DNA polymerase delta strands both manufactures throughout break-induced duplication. Mol. Cell 76, 371– 381 e374 (2019 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Layer, J. V. et al. Polymerase delta promotes chromosomal rearrangements and inaccurate double-strand break repair work. Proc. Natl Acad. Sci. U.S.A. 117, 27566– 27577 (2020 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Wood, R. D. & & Burki, H. J. Repair ability and the cellular age action for killing and anomaly induction after UV. Mutat. Res. 95, 505– 514 (1982 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Lange, S. S., Tomida, J., Boulware, K. S., Bhetawal, S. & & Wood, R. D. The polymerase activity of mammalian DNA Pol zeta is particularly needed for cell and embryonic practicality. PLoS Genet. 12, e1005759 (2016 ).

    Article
    PubMed
    PubMed Central

    Google Scholar

  • Zatreanu, D. et al. Poltheta inhibitors generate BRCA-gene artificial lethality and target PARP inhibitor resistance. Nat. Commun. 12, 3636 (2021 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Kaminski, A. M. et al. Analysis of varied double-strand break synapsis with Pollambda exposes basis for special substrate uniqueness in nonhomologous end-joining. Nat. Commun. 13, 3806 (2022 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Masuda, Y. et al. Characteristics of human duplication consider the elongation stage of DNA duplication. Nucleic Acids Res. 35, 6904– 6916 (2007 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Luedeman, M. E. et al. Poly( ADP) ribose polymerase promotes DNA polymerase theta-mediated end signing up with by activation of end resection. Nat. Commun. 13, 4547 (2022 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Holden, S. J., Uphoff, S. & & Kapanidis, A. N. DAOSTORM: an algorithm for high-density super-resolution microscopy. Nat. Techniques 8, 279– 280 (2011 ).

    Article
    CAS
    PubMed

    Google Scholar

  • Huang, F. et al. Video-rate nanoscopy utilizing sCMOS camera-specific single-molecule localization algorithms. Nat. Techniques 10, 653– 658 (2013 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Huang, F., Schwartz, S. L., Byars, J. M. & & Lidke, K. A. Simultaneous multiple-emitter fitting for single particle super-resolution imaging. Biomed. Decide. Express 2, 1377– 1393 (2011 ).

    Article
    PubMed
    PubMed Central

    Google Scholar

  • Yin, Y., Lee, W. T. C. & & Rothenberg, E. Ultrafast information mining of molecular assemblies in multiplexed high-density super-resolution images. Nat. Commun. 10, 119 (2019 ).

    Article
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Yin, Y. et al. A basal-level activity of ATR links duplication fork security and tension action. Mol. Cell 81, 4243– 4257. e4246 (2021 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Sengupta, P. et al. Penetrating protein heterogeneity in the plasma membrane utilizing PALM and set connection analysis. Nat. Techniques 8, 969– 975 (2011 ).

    Article
    CAS
    PubMed
    PubMed Central

    Google Scholar

  • Veatch, S. L. et al. Connection functions measure super-resolution images and quote evident clustering due to over-counting. PLoS ONE 7, e31457 (2012 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Lee, W. T. C. et al. Single-molecule imaging exposes duplication fork paired development of G-quadruplex structures prevents regional duplication tension signaling. Nat. Commun. 12, 2525 (2021 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

  • Yin, Y. & & Rothenberg, E. Probing the spatial company of molecular complexes utilizing triple-pair-correlation. Sci. Rep. 6, 30819 (2016 ).

    Article
    CAS
    PubMed
    PubMed Central
    ADS

    Google Scholar

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